Tsingke tse005 2×t5 super pcr mix colony
WebMar 5, 2024 · Then, colony PCR was used to identify the bacterial strain. Briefly, about 2 μL of bacteria were picked into a 50 μL 2 × T5 Super PCR Mix (Colony) system (TSINGKE Biological Technology, Beijing, China) and then amplified and sequenced using primers 27F (5′-AGAGTTTGATCCTGGCTCAG-3′) and 1492R (5′-GGTTACCTTGTTA CGACTT-3′). WebThe SuperMix is provided at 2× concentration and can be used at 1× concentration by adding template, primers and H 2 O. • TransTaq ® High Fidelity (HiFi) PCR SuperMix offers 18 …
Tsingke tse005 2×t5 super pcr mix colony
Did you know?
Web丁香通为您找到202条pcr一侧产物出现套峰厂家的工商注册年份、员工人数、年营业额、信用记录、主营产品、相关pcr一侧产物出现套峰产品的供求信息、交易记录等企业详情。丁香通为买家提供用户服务,诚信保障等服务,批发采购pcr一侧产物出现套峰,上丁香通。 WebApr 13, 2024 · The 1.1×T3 Super PCR Mix (TsingKe, Nanjing, China) was used for PCR. The 10% vertical polyacrylamide electrophoresis was performed to observe the cDNA PCR products. AceQ qPCR SYBR Green Master Mix (Vazyme) was used for qRT-PCR, and GAPDH was used to normalize the level of PD-L1-lnc and PD-L1 mRNA. Primers were listed in …
WebJul 18, 2024 · Polymerase Chain Reaction (PCR) was performed in 12 μl reaction volumes containing 2 template DNA, 5 μl of 2 × T5 Super PCR Mix (PAGE; TSINGKE, Beijing, China), 1 μl of 10 μmol/μL primer pairs, and 4 μl of double distilled H 2 O. Target DNA segments were amplified with the following program, 95°C for 5 min, followed by 35 cycles of 95°C for 10 … Web2 ×T5 Super PCR Mix (Colony) 5. Initial denaturation. 98. 30s. 1 × . Forward primer . 0.2. Denaturation. 98. 10s. 29 × . Feverse primer . 0.2. Annealing. T m-5. 30s. Template. 2. …
WebPublish time 2024-09-12 17:24. TOROGreen® Yeast colony PCR Kit is used to amplify fragments from the yeast genome as well as transformed plasmids. The same as E … Web2 O PCR primers OneTaq Master Mix 25 µl PCR Primer 200 nM H 2 O to 50 µl Note: If OneTaq Hot Start Quick-Load 2X Master Mix is used, reactions can be set up at room temperature. If OneTaq Quick-Load 2X Master Mix is used, reactions should be set up on ice. INITIAL DENATURATION PCR Primer 2 minutes 30 CYCLES 94°C 15–30 seconds …
Web1.产品不推荐用于菌落和菌落PCR,如有需要推荐使用我公司专用PCR Mix(产品货号:TSE005)。 2.扩增脏模板时,可通过“稀释模板,增加循环数”或“减少模板量,扩大体 …
t tf 単位Web2 O PCR primers OneTaq Master Mix 25 µl PCR Primer 200 nM H 2 O to 50 µl Note: If OneTaq Hot Start Quick-Load 2X Master Mix is used, reactions can be set up at room … phoenix cash \u0026 carryWebMar 7, 2024 · Pipette 8 µL of LB into PCR tubes and 5 mL (+ antibiotics) into glass culture tubes. Pick a colony off the agar plate and mix it into the LB in a PCR tube. Transfer 5 µL … ttg and gliadinWeb2.5 TaqMan real-time RT-PCR assay. The novel PCR assay was developed by employing the 2×T5 Fast qPCR Mix (TSE301, TsingKe Biotech, China) on CFX384 Touch Real-Time PCR … phoenix catchphraseWebPick one colony and dissolve in 100 μL media 2 . Take 1 μL as DNA sample for PCR 3 . ... Dream Taq PCR Master Mix(2X) 12.5 Forward Primer 0.1-1.uM Reverse primer 0.1-1.uM … phoenix catalogoWeb4 Use 1 µl of reaction for colony PCR (T5 Taq Polymerase) 5 ... Concentration 2XT5 Super PCR Mix 5ul 1x 10uM Forward Primer 0.4ul 0.4uM 10uM Reverse Primer 0.4ul 0.4uM Template DNA 1ul <1ug phoenix casino hotel dealsWeb2×T5 Super PCR Mix (Colony) TSE005 产品说明: 目录号: T5 DNA 聚合酶是新型DNA 聚合酶,由经过改造的Taq DNA 聚合酶融合Sso7d DNA结合域而得来,具有极高的扩增 速度 … ttg atlas copco